The Study of Possible Mechanism of Myosin II inhibited Cytokinesis of Mammalian Cells

Xiaona Li, Meiwen An, Li Wang, Wenzhou Wu, Weiyi Chen

doi:10.6052/0459-1879-2009-3-2008-656

Volume 41 Issue 3

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Article Navigation > Chinese Journal of Theoretical and Applied Mechanics > 2009 > 41(3): 389-398

Xiaona Li, Meiwen An, Li Wang, Wenzhou Wu, Weiyi Chen. The Study of Possible Mechanism of Myosin II inhibited Cytokinesis of Mammalian Cells[J]. Chinese Journal of Theoretical and Applied Mechanics, 2009, 41(3): 389-398. doi: 10.6052/0459-1879-2009-3-2008-656

Citation:

Xiaona Li, Meiwen An, Li Wang, Wenzhou Wu, Weiyi Chen. The Study of Possible Mechanism of Myosin II inhibited Cytokinesis of Mammalian Cells[J]. Chinese Journal of Theoretical and Applied Mechanics, 2009, 41(3): 389-398. doi: 10.6052/0459-1879-2009-3-2008-656

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The Study of Possible Mechanism of Myosin II inhibited Cytokinesis of Mammalian Cells

doi: 10.6052/0459-1879-2009-3-2008-656

Xiaona Li, Meiwen An, Li Wang, Wenzhou Wu, Weiyi Chen

Institute of Applied Mechanics and Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, ChinaInstitute of Applied Mechanics and Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, ChinaInstitute of Applied Mechanics and Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, ChinaInstitute of Applied Mechanics and Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, ChinaInstitute of Applied Mechanics and Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, China

Received Date: 2008-10-23
Rev Recd Date: 2009-01-08
Publish Date: 2009-05-18

Abstract

Abstract

Adherent mammalian cells experience a series of elaborate,but simple morphological changes during cell division. This complex processwas mediated by mechanics and biological molecules. As a kind of motorproteins, myosin II plays an important role in cell division. Recentinvestigations showed that cells on substrates in the absence of myosin IIalso could finish cytokinesis. However, its mechanism is still unclear.In this study, morphology of NRK cells treated with 0.3%DMSO and 30$\mu$mol blebbistatin (myosin II inhibitor) were investigated quantitatively byusing micro-image collecting and analysis system. Intercellular bridgedynamics was simulated using mechanical model of intercellular bridgethinning coupled with mechanical parameters of NRK cells. The distributionof actin in early cytokinesis was examined using immunofluorescence method.Our results showed that intercellular bridge thinning trajectory andmorphology of cells treated with 30 mol blebbistatin were fundamentallydifferent from cells treated with 0.3%DMSO. The surface tensioncontributed greatly to the trajectory of 30 mol blebbistatin group fromfluid mechanical considerations and the measured mechanical parameters.Immunofluorescence results indicated that a mass of actin was recruited tothe fan-shaped leading lamellipodia of daughter cells from the cytoplasm incells treated with 30 mol blebbistatin.Our study indicated that Laplace pressure determined by mechanicalproperties and the motility of cell orchestrated to dictate awell-controlled cytokinesis of cells in the absence of myosin II.
- Normal rat kidney epithelial cells,
- intercellular bridge,
- myosin II,
- mechanical properties,
- morphology