MECHANOTRANSDUCTION OF THE CELL AND ITS PRIMARY CILIUM IN THE MICROFLUIDIC CHANNEL

Li Chaoxin; Wu Xiaogang; Sun Yuqin; Qin Yingze; Duan Wangping; Zhang Meizhen; Wang Yanqin; Chen Weiyi; Wei Xiaochun

doi:10.6052/0459-1879-20-283

Volume 53 Issue 1

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Chinese Journal of Theoretical and Applied Mechanics > 2021 > 53(1): 260-277. > DOI: 10.6052/0459-1879-20-283 CSTR: 32045.14.0459-1879-20-283

Li Chaoxin, Wu Xiaogang, Sun Yuqin, Qin Yingze, Duan Wangping, Zhang Meizhen, Wang Yanqin, Chen Weiyi, Wei Xiaochun. MECHANOTRANSDUCTION OF THE CELL AND ITS PRIMARY CILIUM IN THE MICROFLUIDIC CHANNEL[J]. Chinese Journal of Theoretical and Applied Mechanics, 2021, 53(1): 260-277. DOI: 10.6052/0459-1879-20-283

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MECHANOTRANSDUCTION OF THE CELL AND ITS PRIMARY CILIUM IN THE MICROFLUIDIC CHANNEL

Li Chaoxin^*,
Wu Xiaogang^†,**,2, ,,
Sun Yuqin^†,
Qin Yingze^**,
Duan Wangping^**,
Zhang Meizhen^††,
Wang Yanqin^†,
Chen Weiyi^†,3), ,,
Wei Xiaochun^**

^* College of Mechanical and Vehicle Engineering, Taiyuan University of Technology, Taiyuan 030024, China
^† College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, China
^** Shanxi Provincial Key Laboratory for Repair of Bone and Soft Tissue Injury, Taiyuan 030001, China
^†† Physical Education College of Taiyuan University of Technology, Taiyuan 030024, China

Received Date: August 12, 2020

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Abstract

Cells usually live in a complex physiological environment. The primary cilium, which is a an important organelle of the cell, is attached to the cell surface and is regard as an important mechanical signal sensor to help living cell receive various external mechanical signals, the primary cilium is considered to be closely related to physiological activities such as metabolism, development, division and proliferation of the living cell. In order to study the mechanotransduction behavior of the living cell and the primary cilium growing on its surface in a microfluidic environment, this paper established the adherent cells within a rectangular microfluidic control channel model system, cells with poroviscoelastic properties are in a culture solution driven by the pressure gradient and electric field driven loads. The mechanical signal responses of cytoplasm and nucleus of cells such as the stress, strain, pore fluid pressure and pore fluid velocity under oscillatory laminar flow were investigated, as the mechanical signal's receptors of the living cell, the primary cilium's biomechanical behavior was quantitatively investigated. The results show that the mechanical response of the living cell under an oscillating laminar flow field has the same oscillating law as the synchronous external the pressure gradient and electric field driven loads. The permeability of the living cell is one of the most important physical parameters affecting the cell's poroviscoelastic behavior. Primary cilium is the main mechanoreceptor organelle. The living cell can adjust their mechanical sensitivity (stress-affected zone) by changing the length and diameter of its primary cilium. With the increase of the length of the primary cilium, the flexural rigidity of the primary cilium decreases, but the sensitivity increases. The establishment of the model provides a basis for further research on the microscopic mechanisms of cell growth and differentiation under the loading of microfluidic shear stress, and also provides theoretical technical support for testing the microstructure mechanical properties of the cell anticipates (protein chains such as primary cilium).
- cell,
- primary cilium,
- microfluidic control,
- poroviscoelasticity,
- mechanotransduction

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References

[1]	吕永钢, 詹世革 . 第三届全国生物力学青年学者学术研讨会报告综述. 力学学报, 2019,51(1):306-311. (L $ddot{u}$ Yonggang, Zhan Shige . Review of the third national symposium on biomechanic. Chinese Journal of Theoretical and Applied Mechanics 2019,51(1):306-311 (in Chinese))
[2]	林金明 . 微流控芯片细胞分析. 北京: 科学出版社, 2018. (Lin Jinming. Microfluidic Chip Cell Analysis. Beijing: Science Press, 2018 (in Chinese))
[3]	Du J, Liu X, Xu X . Advances in isolation and enrichment of circulating tumor cells in microfluidic chips. Chinese Journal of Chromatography 2014,32(1):7-12
[4]	Hung PJ, Lee PJ, Sabounchi P , et al. Continuous perfusion microfluidic cell culture array for high-throughput cell-based assays. Biotechnology & Bioengineering 2005,89(1):1-8
[5]	Singh S, Melnik R . Coupled thermo-electro-mechanical models for thermal ablation of biological tissues and heat relaxation time effects. Physics in Medicine and Biology 2019,64(24):245008
[6]	Visone R, Giuseppe , Occhetta P , et al. A microscale biomimetic platform for generation and electro-mechanical stimulation of 3D cardiac microtissues. Apl Bioengineering 2018,2(4):046102
[7]	Qi H, Xu M . Unsteady flow of viscoelastic fluid with fractional Maxwell model in a channel. Mechanics Research Communications 2007,34(2):210-212
[8]	Chaube MK, Tripathi D, Anwar BO , et al. Peristaltic creeping flow of power law physiological fluids through a nonuniform channel with slip effect. Applied Bionics and Biomechanics 2015,64(24):152802-152802
[9]	王兆伟 . 力-电协同驱动细胞培养腔内微液流动的理论模拟研究. [硕士论文]. 太原: 太原理工大学, 2018. (Wang Zhaowei . Theoretical simulation study of force-electricity cooperation driving the micro-liquid flow in the cell culture cavity. [Master thesis]. Taiyuan: Taiyuan University of Technology, 2018 (in Chinese))
[10]	Guilak F, Haider MA, Setton LA , et al. Multiphasic models of cell mechanics. Cytoskeletal Mechanics 2006,5:84-102
[11]	Liu Y, Mollaeian K, Ren JJM , et al. Finite element modeling of living cells for afm indentation-based biomechanical characterization. Micron (Oxford, England: 1993) 2018,116:108-115
[12]	Hoang MT, Bonnet G, Perrot C . Multi-scale acoustics of partially open cell poroelastic foams. Journal of the Acoustical Society of America 2013,133(5):3289
[13]	裘钧 . 骨细胞力学的实验和数值研究. [博士论文]. 北京: 清华大学, 2011. (Qiu Jun . Experimental and numerical research on bone cell mechanics. [PhD Thesis]. Beijing: Tsinghua University, 2011 (in Chinese))
[14]	Islam MD, Raffaella R . An analytical poroelastic model of a spherical tumor embedded in normal tissue under creep compression. Journal of Biomechanics 2019,89:48-56
[15]	Zhang D . Oscillatory pressurization of an animal cell as a poroelastic spherical body. Annals of Biomedical Engineering 2005,33(9):1249
[16]	武晓刚, 于纬伦, 王兆伟等. 一种骨小管中液体流动产生的流量及切应力模型. 力学学报, 2016,48(5):1208-1216. (Wu Xiaogang, Yu Weilun, Wang Zhaowei , et al. A model of flow and shear stress generated by fluid flow in bone tubules. Chinese Journal of Theoretical and Applied Mechanics 2016,48(5):1208-1216 (in Chinese))
[17]	Mollaeian K, Liu Y, Bi S , et al. Atomic force microscopy study revealed velocity-dependence and nonlinearity of nanoscale poroelasticity of eukaryotic cells. J Mech Behav Biomed Mater 2018,78:65-73
[18]	Wei F, Lan F, Liu B , et al. Poroelasticity of cell nuclei revealed through atomic force microscopy characterization. Applied Physics Letters 2016,109(21):7159
[19]	Bangs F, Anderson KV . Primary cilia and mammalian hedgehog signaling. Cold Spring Harbor Perspectives in Biology 2016,9(5):a028175
[20]	Berbari NF, Connor AK, Haycraft CJ , et al. The primary cilium as a complex signaling center. Current Biology 2009,19(13):R526-R535
[21]	Eichholz KF, Hoey DA . The Role of the Primary Cilium in Cellular Mechanotransduction. Mechanobiology 2016: 61-73
[22]	Lim YC, Cooling MT, Long DS . Computational models of the primary cilium and endothelial mechanotransmission. Biomechanics and Modeling in Mechanobiology 2015,14(3):665-678
[23]	Spasic M, Jacobs CR . Primary cilia: Cell and molecular mechanosensors directing whole tissue function. Seminars in Cell & Developmental Biology 2017,71:42-52
[24]	Khayyeri H, Barreto S, Lacroix D . Primary cilia mechanics affects cell mechanosensation: a computational study. Journal of Theoretical Biology 2015,379:38-46
[25]	Moore ER, Xing ZY, Seul RH , et al. Periosteal progenitors contribute to load-induced bone formation in adult mice and require primary cilia to sense mechanical stimulation. Stem Cell Research & Therapy 2018,9(1):190
[26]	Goetz SC, Anderson KV . The primary cilium: A signalling centre during vertebrate development. Nature Reviews Genetics 2010,11(5):331-344
[27]	Temiyasathit S, Jacobs CR . Osteocyte primary cilium and its role in bone mechanotransduction. Chemistry of Life 2010,1192(1):422-428
[28]	Luu VZ, Biswajit C, Mohammed AO , et al. Role of endothelial primary cilia as fluid mechanosensors on vascular health. Atherosclerosis 2018,275:196
[29]	Ahern DP, Thompson CL, Duffy MP , et al. Primary Cilia Mechanobiology. Mechanobiology 2020: 99-115
[30]	Buljan VA, Graeber MB, Holsinger RM , et al. Calcium--axonemal microtubuli interactions underlie mechanism(s) of primary cilia morphological changes. Journal of Biological Physics 2017,44:53-80
[31]	Flaherty J, Feng Z, Peng Z , et al. Primary cilia have a length-dependent persistence length. Biomechanics and Modeling in Mechanobiology 2020,19(2):445-460
[32]	Blythman R, Persoons T, Jeffers N , et al. Localised dynamics of laminar pulsatile flow in a rectangular channel. International Journal of Heat & Fluid Flow 2017,66(8):8-17
[33]	王兆伟, 武晓刚, 陈魁俊等. 一种力-电协同驱动的细胞微流控培养腔理论模型. 力学学报, 2018,50(1):124-137. (Wang Zhaowei, Wu Xiaogang, Chen Kuijun , et al. A theoretical microfluidic flow model for the cell culture chamber under the pressure gradient and electric field driven loads. Chinese Journal of Theoretical and Applied Mechanics 2018,50(1):124-137 (in Chinese))
[34]	Miller AD, Chama A, Louw TM , et al. Frequency sensitive mechanism in low-intensity ultrasound enhanced bioeffects. PloS One 2017,12(8):e0181717
[35]	陈魁俊 . 骨单元到骨细胞尺度的多孔弹性力学信号传导. [硕士论文]. 太原: 太原理工大学, 2019. (Chen Kuijun . Signal transduction of poroelasticity from bone unit to bone cell scale. [Master Thesis]. Taiyuan: Taiyuan University of Technology, 2019 (in Chinese))
[36]	Rydholm S, Zwartz G, Kowalewski J , et al. Mechanical properties of primary cilia regulate the response to fluid flow. American Journal of Physiology-renal Physiology 2010,298(5):1096-1102
[37]	Molla-Herman A, Ghossoub R, Blisnick T , et al. The ciliary pocket: An endocytic membrane domain at the base of primary and motile cilia. Journal of Cell Science 2010,123(10):1785-1795
[38]	于纬伦, 武晓刚, 李朝鑫等. 骨陷窝-骨细胞形状和方向对骨单元内液体流动行为的影响. 力学学报, 2020,52(3):244-254. (Yu Weilun, Wu Xiaogang, Li Chaoxin , et al. Effect of osteocyte-lacunae shape and direction on the fluid flow behavior in osteon. Chinese Journal of Theoretical and Applied Mechanics 2020,52(3):244-254 (in Chinese))
[39]	冯世亮, 周吕文, 吕守芹等. 悬浮态上皮细胞粘附的力学-化学耦合模型及数值模拟. 力学学报, 2020,52(3):255-264. (Feng Shiliang, Zhou Lvwen , L $ddot{u}$ Shouqin, et al. Mechanochemical coupling model and numerical simulation for cell-cell adhesion in suspended epithelial cells. Chinese Journal of Theoretical and Applied Mechanics 2020,52(3):255-264 (in Chinese))
[40]	Vaughan TJ, Mullen CA, Verbruggen SW , et al. Bone cell mechanosensation of fluid flow stimulation: A fluid--structure interaction model characterising the role integrin attachments and primary cilia. Biomechanics & Modeling in Mechanobiology 2015,14(4):703-718
[41]	Boehlke C, Kotsis F, Patel V , et al. Primary cilia regulate mtorc1 activity and cell size through lkb1. Nature Cell Biology 2010,12(11):1115-1122
[42]	Tao F, Jiang T, Tao H , et al. Primary cilia: versatile regulator in cartilage development. Cell Proliferation 2020,2:1-12
[43]	Jamal MH, Nauli SM , The effect of primary cilia restoration on cancer cells. The FASEB Journal. 2020,34(S1):1-1
[44]	Mcglashan SR, Cluett EC, Jensen CG , et al. Primary cilia in osteoarthritic chondrocytes: from chondrons to clusters. Developmental Dynamics An Official Publication of the American Association of Anatomists 2008,237(8):2013-2020
[45]	Jacobs C, Spasic M. Regulation of gene expression by modulating primary cilia length. US: US20190015423, 2019 -01-17
[46]	Schwartz EA, Leonard ML, Bizios R , et al. Analysis and modeling of the primary cilium bending response to fluid shear. American Journal of Physiology 1997,272(2):132-138

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MECHANOTRANSDUCTION OF THE CELL AND ITS PRIMARY CILIUM IN THE MICROFLUIDIC CHANNEL

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