Abstract: Numerical modeling with experimental analysis isincreasingly being used to evaluate the mechanical properties of livingcells in single cell mechanics. In the present study, through the continuummechanics process, a new constitutive model for cardiac myocyte isdeveloped, and together with the micropipette aspiration experiment, themechanical property of cardiac myocyte is investigated. The commonly usedstandard linear solid model is extended into a nonlinear viscoelasticconstitutive model in which was introduced the hyperelasticity to describethe large deformation of myocytes in response to micropipette aspiration.Based on the constitutive model and experiment data, the experiment processis simulated, and the computational results are compared with the experimentresults and classic theoretical solutions. The results show that the newconstitutive model suits the computation of large deformation of cells.