脱细胞真皮基质在角膜中植入性的生物力学研究

汲婧; 刘子源; 张晶; 李学民; 王薇

doi:10.6052/0459-1879-13-439

脱细胞真皮基质在角膜中植入性的生物力学研究

doi: 10.6052/0459-1879-13-439

1.
北京航空航天大学生物与医学工程学院, 生物力学与力生物学教育部重点实验室, 北京100191
2. 北京大学第三医院北京大学眼科中心, 视觉损伤与修复教育部重点实验室, 北京100083

基金项目: 首都科学发展基金资助项目（D101100050010035）.

详细信息

作者简介:
王薇，教授，主要研究方向：眼视光学、眼病理、眼组织工程、干细胞.

中图分类号: Q819
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出版历程
- 收稿日期: 2013-12-20
- 修回日期: 2014-01-02
- 刊出日期: 2014-01-18

BIOMECHANICAL INVESTIGATION ON HOST RESPONSE OF ACELLULAR DERMAL MATRIX IN LAMELLAR CORNEAL TRANSPLANTATION

1.
Key Laboratory for Biomechanics and Mechanobiology, Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China
2. Key Laboratory of Vision Loss and Restoration, Ministry of Education, Department of Ophthalmology, Peking University Third Hospital, Peking University Eye Center, Beijing 100083, China

Funds: The project was supported by the Capital Clinical Application Research Project (D101100050010035).

摘要

摘要: 对脱细胞真皮基质（acellular dermal matrix, ADM）囊袋植入兔角膜后的生物相容性、生物学转归及组织结构改变进行生物力学研究，以探讨其作为组织工程角膜支架材料的可行性. 对ADM 进行了系统的组织微观结构观察和植入性实验研究，组织结构观察包括HE 染色、胶原染色、免疫荧光染色和透射电镜观察. 在植入实验中，将ADM 按不同预处理方法分为无干预组、脱水组和复水组，将3 组ADM 囊袋植入兔角膜，观察其术后不同时期在体和离体的生物相容性和组织学特性. 结果发现：ADM 由横纵交替、排列疏松的Ⅰ和Ⅲ型胶原纤维束构成，未见细胞结构；囊袋植入角膜后有不同程度的炎症反应，部分植片透明；组织学观察显示3 组ADM植入角膜后均有细胞长入，并进行了胶原纤维重塑. 研究工作表明，经适当预处理且结构致密的脱细胞真皮基质植入后可以长期稳定存留于角膜内，是一种比较理想的组织工程角膜支架.
- 眼生物力学 /
- 脱细胞真皮基质 /
- 生物相容性 /
- 角膜基质支架 /
- 角膜组织工程
Abstract: The biocompatibility, biological changes and tissue structure of acellular dermal matrix (ADM) in lamellar corneal transplantation (LCT) as a substitute are experimentally investigated in order to evaluate its possibility as a supporting material in corneal tissue engineering. The microstructure of ADM was observed with light microscope (LM), fluorescence microscope, polarizing microscope, and transmission electron microscope (TEM). In the implantation experiment, the ADM was processed with 3 different methods and implanted into rabbit corneal stroma. The ocular surface condition was observed with slit lamp microscope (SLM) postoperatively. The rabbits were sacrificed and morphology structures of the corneal grafts were studied by LM and polarizing microscope at 1 week, 4 weeks, 12 weeks and 24 weeks after transplantation. It was found that the ADM were composed mainly of Type-I and III collagens organized loosely and disorderly. No cellular structure was observed. There were inflammatory responses after implantation. Some implants turned to be transparent. Histological study evidenced both the growth of cells into the implant and the reconstruction collagen fiber. The results showed that the ADM with compact structure and proper pretreatment could stably exist in corneal stroma after implantation and, therefore, could be used as a potential scaffold in corneal tissue engineering.
- ocular biomechanics /
- acellular dermal matrix /
- biocompatibility /
- corneal stroma scaffold /
- corneal tissue engineering }

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