Abstract: Displacement efficiency and displacement pattern of multiphase flow in porous media have a profound influence on many geo-energy applications such as geologic CO_2 sequestration and enhanced oil recovery. Wettability is one of the most important factors affecting the displacement pattern and displacement efficiency of multiphase flow in porous media. Here, we combined the glass microfluidics, inverted microscope and high speed CMOS camera to set up a visualization experimental system and modified the surface wettability of the glass microfluidics by using the silanization treatment and piranha solution. Pore-scale visualization displacement experiments were conducted on five flow rates and two wetting conditions (hydrophilic and hydrophobic conditions) in glass microfluidics which are fabricated from the pore structure of natural sandstone. Experimental results show that the displacement pattern shifts from capillary fingering to compact displacement pattern both in the hydrophilic and hydrophobic media as the flow rate increases. Under lower flow rates, the capillary force plays the dominant role in the fluid invasion processes. The invasion finger width and the number of air clusters in hydrophilic media are both smaller than those in the hydrophobic media, but the maximum air cluster radius, the average cluster radius and the standard deviation of cluster radius are all greater under hydrophilic conditions. The results also demonstrate that the displacement efficiency under hydrophilic condition is significantly lower than that of hydrophobic conditions due to single-channel flow and "by pass" flow phenomena which both only occur in hydrophilic media. Finally, a modified capillary number was introduced in order to consider the role of wettability (contact angle) under favourable displacement. Then, a relationship between the displacement efficiency and the modified capillary number was proposed, which provides a potential and useful method for the prediction of displacement efficiency under different wetting conditions during favourable displacement.