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申峰, 张杰, 艾明珠, 张越东, 刘赵淼. 微流控粒子分选中圆形微凹槽容纳特性研究. 力学学报, 待出版. DOI: 10.6052/0459-1879-24-600
引用本文: 申峰, 张杰, 艾明珠, 张越东, 刘赵淼. 微流控粒子分选中圆形微凹槽容纳特性研究. 力学学报, 待出版. DOI: 10.6052/0459-1879-24-600
Shen Feng, Zhang Jie, Ai Mingzhu, Zhang Yuedong, Liu Zhaomiao. Study on the holding characteristics of round microcavities for particle sorting using microfluidics. Chinese Journal of Theoretical and Applied Mechanics, in press. DOI: 10.6052/0459-1879-24-600
Citation: Shen Feng, Zhang Jie, Ai Mingzhu, Zhang Yuedong, Liu Zhaomiao. Study on the holding characteristics of round microcavities for particle sorting using microfluidics. Chinese Journal of Theoretical and Applied Mechanics, in press. DOI: 10.6052/0459-1879-24-600

微流控粒子分选中圆形微凹槽容纳特性研究

STUDY ON THE HOLDING CHARACTERISTICS OF ROUND MICROCAVITIES FOR PARTICLE SORTING USING MICROFLUIDICS

  • 摘要: 微流控技术由于具备操控微通道中微小体积流体的能力, 已成为操控粒子和细胞的新平台. 基于粒子惯性迁移和微凹槽涡胞捕获的粒子分选方法, 是一种重要的微流控粒子操控技术. 目前, 微凹槽容纳的粒子数量不高, 制约了该方法的效率. 为了提高微凹槽粒子容量, 对圆形微凹槽进行结构设计, 并利用高速显微成像技术和数值模拟, 研究了不同圆形微凹槽的粒子容纳能力. 研究发现, 相同入口雷诺数(Re = 37 ~ 555)下, 带底腔的圆形微凹槽相较于普通圆形微凹槽容纳的粒子数量提升了45%, 这是因为增加底腔后使得涡流线向下延展, 形成更深的“U形”结构, 可以容纳更多的粒子; 当Re = 482时, 带底腔的直径500 μm的圆凹槽比直径600 μm的圆凹槽的粒子容量提高了93.9%, 原因是前者凹槽内粒子运动轨道与流线更加吻合, 粒子轨道面积与凹槽面积占比达到了97%; 随着Re增加, 从侧通道收集到的粒子富集浓度整体呈现先缓慢增大后减小的趋势, 收集到的20 μm粒子的最大富集浓度为初始悬浮液的126.7倍; 不同微凹槽内粒子群的轨道运动受到涡流场特性、粒子物性及间相互作用和壁面限制作用等因素的共同影响. 研究结果对微凹槽结构设计、提高粒子分选性能有重要指导意义.

     

    Abstract: Due to its capability to manipulate small volume fluids in microchannels, microfluidics has emerged as a novel platform for the manipulation of particles and cells. The method of using inertial migration and vortex trapping of particles in microcavities has become an important microfluidic particle manipulation technique. Currently, the number of captured particles accommodated by the microcavity is not high, which restricts the sorting efficiency of this method. To improve the holding capacity of microcavities, different round microcavities were designed, and their holding characteristics for sorted particles were investigated by using high-speed microimaging technology and numerical simulation. The results show that under the same inlet Reynolds number (Re = 37 ~ 555), the number of captured particles in a round microcavity with a bottom chamber can be enhanced by 45% compared with that in an ordinary round microcavity. The reason is that the addition of the bottom chamber causes the vortex streamlines to extend downward, creating a deeper "U-shaped" structure to hold more particles. At Re = 482, the holding capacity in a round cavity with a diameter of 500 μm increases by 93.9% compared to that with a diameter of 600 μm. The reason is that the particle motion track in the round microcavity with a diameter of 500 μm is more consistent with the streamlines, and the ratio of particle trajectory area to the cavity area is up to 97%. The concentration of particles collected from the side channel shows an overall trend of slowly increasing and then decreasing with increasing Re. The maximum concentration of 20 μm particles is 126.7 times higher than that in the initial suspension. The orbital motion of particles in different cavities is influenced by the vortex flow field characteristics, particle properties, particle interactions and wall confined effect. The research results could provide useful guidance for the design of microcavity structures and the improvement of particle sorting performance.

     

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