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中文核心期刊

周期性拉伸不同参数对肺腺癌细胞增殖的影响

Effects of mechanical strain different load parameters on proliferation of lung adenocarcinoma cell in vitro

  • 摘要: 通过FX-4000基底加载系统研究了在不同波形、频率、循环数及拉伸幅度下周期性拉伸应变对人肺腺癌A549细胞株生长增殖的影响,应用Image-pro软件对A549细胞的增殖动力学变化进行了分析. 实验表明:(1) 在laminin基底膜上,应变0\%\sim15\%, 加载时间2\,h, 方波拉伸组细胞增殖率(PR)明显降低,正弦波、心形波及三角波拉伸组细胞PR与对照组比较无明显差异;(2) 方波作用下, 0.5\,Hz及1.0\,Hz频率组细胞增殖明显受到抑制, 0.3\,Hz及0.7\,Hz频率组细胞PR与对照组比较无明显差异;(3) 方波作用下,幅度为15\%, 20\%及25\%的拉伸组与对照组比较细胞PR均无明显差异. 研究表明:A549细胞株对体外的生理应变作出响应时,方波作用下1.0\,Hz频率组的抑制作用最明显,且加载时间越长抑制作用越强.

     

    Abstract: The effects of waveform, frequency, cycle number andelongation on proliferation of human lung adenocarcinoma cell line A549 werestudied by FX-4000 strain unit. Image analysis revealed that cellularproliferation rate(PR) reduced significantly at squarewave group, The PR hadno distinct difference at heartwave, trianglewave and sinewave groupcompared with control after A549 cells were subjected to 0\%\sim 15\%elongation for 2\,h. The PR reduced significantly at 0.5\,Hz and 1.0\,Hzgroup, ThePR had no distinct difference at 0.3\,Hz and 0.7\,Hz group after A549 cells weresubjected to squarewave for 2\,h. The PR had no distinct difference at15\%, 20\% and 25\% elongation for squarewave group. It indicates thatA549 cells do respond to physiological strain in vitro. The effects ofsquarewave and 1.0\,Hz tension was distinct for A549 cells proliferation.

     

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