Abstract: The effects of waveform, frequency, cycle number andelongation on proliferation of human lung adenocarcinoma cell line A549 werestudied by FX-4000 strain unit. Image analysis revealed that cellularproliferation rate(PR) reduced significantly at squarewave group, The PR hadno distinct difference at heartwave, trianglewave and sinewave groupcompared with control after A549 cells were subjected to 0\%\sim 15\%elongation for 2\,h. The PR reduced significantly at 0.5\,Hz and 1.0\,Hzgroup, ThePR had no distinct difference at 0.3\,Hz and 0.7\,Hz group after A549 cells weresubjected to squarewave for 2\,h. The PR had no distinct difference at15\%, 20\% and 25\% elongation for squarewave group. It indicates thatA549 cells do respond to physiological strain in vitro. The effects ofsquarewave and 1.0\,Hz tension was distinct for A549 cells proliferation.